3V62

Structure of the S. cerevisiae Srs2 C-terminal domain in complex with PCNA conjugated to SUMO on lysine 164

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Ubiquitin-like protein SMT3	polymer	84	9882.3	2	UniProt (Q12306) Pfam (PF00240) In PDB	Saccharomyces cerevisiae (Baker's yeast)
2	B, E	Proliferating cell nuclear antigen	polymer	258	28871.9	2	UniProt (P15873) Pfam (PF00705) Pfam (PF02747) In PDB	Saccharomyces cerevisiae (Baker's yeast)	PCNA
3	C, F	ATP-dependent DNA helicase SRS2	polymer	69	7703.8	2	UniProt (P12954) In PDB	Saccharomyces cerevisiae (Baker's yeast)
4	B, E	N-ETHYLMALEIMIDE	non-polymer		125.1	4	Chemie (NEQ)
5	B, E	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
6		water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, D: 20 - 98 (UniProt: Q12306)

PDB	External Database	Details
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Met 18	-	expression tag
Arg 19	-	expression tag

B, E: 1 - 258 (UniProt: P15873)

PDB	External Database	Details
Gly 127	Lys 127	engineered mutation

C, F: 1107 - 1174 (UniProt: P12954)

PDB	External Database	Details
Ser 1106	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	92916.0
Non-Polymers*	Number of molecules	8
	Total formula weight	884.8
All*	Total formula weight	93800.7

*Water molecules are not included.