3V62

Structure of the S. cerevisiae Srs2 C-terminal domain in complex with PCNA conjugated to SUMO on lysine 164

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	C 1 2 1
Unit cell lengths	196.817, 62.256, 139.247
Unit cell angles	90.00, 135.04, 90.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.18702
Rwork	0.184
R-free	0.23656
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1PLQ and 1EUV
RMSD bond length	0.008
RMSD bond angle	1.697
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.066	0.025	0.632
Number of reflections	26583
<I/σ(I)>	11.8
Completeness [%]	99.7	98.9	99.4
Redundancy	3.8	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	279	1.9 M AMMONIUM SULFATE 4% PEG 400 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K