3V5C

Crystal structure of the mutant E234A of Galacturonate Dehydratase from GEOBACILLUS SP. complexed with Mg

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Mandelate racemase/muconate lactonizing protein	polymer	392	43865.2	4	UniProt (D3EID5)	Paenibacillus sp.
2	E, G, H, I, L... (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
3	F, J, K, M, N... (A, B, C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	6	Chemie (PEG)
4	R, S, T, U (A, B, C, D)	water	water		18.0	1498	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 384 (UniProt: D3EID5)

PDB	External Database	Details
Ser 2	-	expression tag
Leu 3	-	expression tag
Ala 234	Glu 232	engineered mutation
Glu 385	-	expression tag
Gly 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	175460.6
Non-Polymers*	Number of molecules	13
	Total formula weight	806.9
All*	Total formula weight	176267.5

*Water molecules are not included.