3V5C
Crystal structure of the mutant E234A of Galacturonate Dehydratase from GEOBACILLUS SP. complexed with Mg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.271, 65.863, 151.218 |
Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
Resolution | 40.466 - 1.530 |
R-factor | 0.1866 |
Rwork | 0.185 |
R-free | 0.21610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p3b |
RMSD bond length | 0.006 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.466 |
High resolution limit [Å] | 1.530 |
Number of reflections | 199609 |
Completeness [%] | 87.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 25% PEG3350, 0.1M Tris, 0.2M sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP |