3V4I

Crystal structure of HIV-1 reverse transcriptase (RT) with DNA and AZTTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	HIV-1 Reverse Transcriptase P66 subunit	polymer	556	64022.4	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 BH10 (HIV-1)
2	B, D (B, D)	HIV-1 Reverse Transcriptase P51 subunit	polymer	428	50039.5	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus type 1 BH10 (HIV-1)
3	E, F (T, E)	DNA (5'-D(*AP*TP*GP*GP*AP*AP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3')	polymer	27	8416.4	2
4	G, H (P, F)	DNA (5'-D(*AP*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*(ATM))-3')	polymer	21	6490.3	2
5	I, K (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	J, L (A, C)	3'-AZIDO-3'-DEOXYTHYMIDINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (AZT)

Sequence modifications

A, C: 1 - 554 (UniProt: P03366)

PDB	External Database	Details
Met -1	-	expression tag
Val 0	-	expression tag
Cys 258	Gln 857	engineered mutation
Ser 280	Cys 879	engineered mutation
Asn 498	Asp 1097	engineered mutation

B, D: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	257937.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1063.0
All*	Total formula weight	259000.2

*Water molecules are not included.