3V4I
Crystal structure of HIV-1 reverse transcriptase (RT) with DNA and AZTTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.053, 132.647, 138.014 |
Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
Resolution | 45.543 - 2.798 |
R-factor | 0.2296 |
Rwork | 0.229 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jsm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.380 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.798 | 2.798 |
Rmerge | 0.083 | 0.523 |
Number of reflections | 73202 | |
<I/σ(I)> | 11.6 | 1.9 |
Completeness [%] | 92.8 | 85.1 |
Redundancy | 3.2 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.8 | 277 | PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE, pH 7.2, EVAPORATION, temperature 277K |