3V44

Crystal structure of the N-terminal fragment of zebrafish TLR5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Toll-like receptor 5b and variable lymphocyte receptor B.61 chimeric protein	polymer	407	45719.0	1	UniProt (B3DIN1) UniProt (Q4G1L2) Pfam (PF13855) Pfam (PF11921)	Danio rerio (zebra fish inshore hagfish)
2	B, C, D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
3	E (A)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

A: 22 - 342 (UniProt: B3DIN1)

PDB	External Database	Details
Ala 18	-	expression tag
Asp 19	-	expression tag
Pro 20	-	expression tag
Gly 21	-	expression tag
Glu 24	Val 24	engineered mutation
Val 124	Leu 124	engineered mutation
Lys 159	Gln 159	engineered mutation
Lys 227	Arg 227	engineered mutation
Thr 229	Ser 229	engineered mutation
Asn 334	Asp 334	engineered mutation

A: 343 - 417 (UniProt: Q4G1L2)

PDB	External Database	Details
Ser 418	-	expression tag
Ala 419	-	expression tag
Ser 420	-	expression tag
Leu 421	-	expression tag
Val 422	-	expression tag
Pro 423	-	expression tag
Arg 424	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45719.0
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	46382.7

*Water molecules are not included.