3V2Y

Crystal Structure of a Lipid G protein-Coupled Receptor at 2.80A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sphingosine 1-phosphate receptor 1, Lysozyme chimera (E.C.3.2.1.17)	polymer	520	58973.2	1	UniProt (P21453) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) In PDB	Homo sapiens (Human)	S1P1,Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1,S1P receptor Edg-1,Lysis protein,Lysozyme,Muramidase
2	A	{(3R)-3-amino-4-[(3-hexylphenyl)amino]-4-oxobutyl}phosphonic acid	non-polymer		342.4	1	Chemie (ML5)
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
4		water	water		18.0	11	Chemie (HOH)

Sequence modifications

A: 2 - 231 (UniProt: P21453)

PDB	External Database	Details
Met -17	-	expression tag
Lys -16	-	expression tag
Thr -15	-	expression tag
Ile -14	-	expression tag
Ile -13	-	expression tag
Ala -12	-	expression tag
Leu -11	-	expression tag
Ser -10	-	expression tag
Tyr -9	-	expression tag
Ile -8	-	expression tag
Phe -7	-	expression tag
Cys -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Phe -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag

A: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1012	Arg 12	conflict
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	conflict

A: 245 - 326 (UniProt: P21453)

PDB	External Database	Details
-	Lys 250	deletion
Asn 251	Ser 251	see remark 999
Val 252	Leu 252	see remark 999
Gly 327	-	expression tag
Arg 328	-	expression tag
Pro 329	-	expression tag
Leu 330	-	expression tag
Glu 331	-	expression tag
Val 332	-	expression tag
Leu 333	-	expression tag
Phe 334	-	expression tag
Gln 335	-	expression tag
Gly 336	-	expression tag
Pro 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
Asp 348	-	expression tag
Tyr 349	-	expression tag
Lys 350	-	expression tag
Asp 351	-	expression tag
Asp 352	-	expression tag
Asp 353	-	expression tag
Asp 354	-	expression tag
Lys 355	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	58973.2
Non-Polymers*	Number of molecules	2
	Total formula weight	563.6
All*	Total formula weight	59536.7

*Water molecules are not included.