3V2Y
Crystal Structure of a Lipid G protein-Coupled Receptor at 2.80A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.940, 69.700, 81.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.520 - 2.800 |
| R-factor | 0.2307 |
| Rwork | 0.229 |
| R-free | 0.27170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7TM of b2AR and T4L |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.650 |
| Data reduction software | XDS |
| Data scaling software | Microdiffaction (data assembly methods) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.160 | 0.420 |
| Number of reflections | 15297 | |
| <I/σ(I)> | 6.1 | 1.9 |
| Completeness [%] | 97.2 | 94.4 |
| Redundancy | 6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Lipid cubic phase | 287 | 0.1M Tricine, 34-36% PEG400, 80mM sodium citrate and 4% glycerol, Lipid cubic phase, temperature 287K | |
| 1 | Lipid cubic phase | 287 | 0.1M Tricine, 34-36% PEG400, 80mM sodium citrate and 4% glycerol, Lipid cubic phase, temperature 287K |
