3V2Y
Crystal Structure of a Lipid G protein-Coupled Receptor at 2.80A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.940, 69.700, 81.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.520 - 2.800 |
R-factor | 0.2307 |
Rwork | 0.229 |
R-free | 0.27170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7TM of b2AR and T4L |
RMSD bond length | 0.010 |
RMSD bond angle | 0.650 |
Data reduction software | XDS |
Data scaling software | Microdiffaction (data assembly methods) |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.160 | 0.420 |
Number of reflections | 15297 | |
<I/σ(I)> | 6.1 | 1.9 |
Completeness [%] | 97.2 | 94.4 |
Redundancy | 6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Lipid cubic phase | 287 | 0.1M Tricine, 34-36% PEG400, 80mM sodium citrate and 4% glycerol, Lipid cubic phase, temperature 287K | |
1 | Lipid cubic phase | 287 | 0.1M Tricine, 34-36% PEG400, 80mM sodium citrate and 4% glycerol, Lipid cubic phase, temperature 287K |