3V20
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Endonuclease Bse634IR | polymer | 293 | 33762.8 | 2 | UniProt (Q8RT53) Pfam (PF07832) | Geobacillus stearothermophilus | |
| 2 | C, D (C, D) | DNA (5'-D(*TP*CP*GP*CP*AP*CP*CP*GP*GP*TP*GP*CP*G)-3') | polymer | 13 | 3968.6 | 2 | synthetic construct | ||
| 3 | E (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | F, H (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 5 | G (A) | 2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL | non-polymer | 310.4 | 1 | Chemie (7PE) | |||
| 6 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 106 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 293 (UniProt: Q8RT53)
| PDB | External Database | Details |
|---|---|---|
| Phe 110 | Leu 110 | SEE REMARK 999 |
| Asp 111 | His 111 | SEE REMARK 999 |
| Ser 130 | Thr 130 | SEE REMARK 999 |
| Ala 226 | Arg 226 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 75462.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 426.0 | |
| All* | Total formula weight | 75888.7 |
