3V20
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGT |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-25 |
| Detector | MAR555 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.937, 87.387, 125.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.556 - 2.350 |
| R-factor | 0.2457 |
| Rwork | 0.240 |
| R-free | 0.29343 |
| Starting model (for MR) | 1knv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.186 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.556 | 2.460 |
| High resolution limit [Å] | 2.336 | 2.340 |
| Rmerge | 0.054 | 0.332 |
| Number of reflections | 30943 | |
| <I/σ(I)> | 22.8 | 2.3 |
| Completeness [%] | 90.6 | 64.5 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 293 | 100mM Na-acetate pH 4.25-5.5, 10mM CaCl2 or CaAc2, 4-8% (w/v) of PEG8000, VAPOR DIFFUSION, temperature 293K |
