3V1H

Structure of the H258Y mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	1-phosphatidylinositol phosphodiesterase	polymer	306	34788.7	1	UniProt (P45723) Pfam (PF00388) In PDB	Staphylococcus aureus subsp. aureus	Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC
2	A	1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE	non-polymer		180.2	1	Chemie (INS)
3	A	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
4		water	water		18.0	265	Chemie (HOH)

Sequence modifications

A: 3 - 302 (UniProt: P45723)

PDB	External Database	Details
Tyr 258	His 268	engineered mutation
Leu 303	-	expression tag
Glu 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34788.7
Non-Polymers*	Number of molecules	3
	Total formula weight	298.2
All*	Total formula weight	35087.0

*Water molecules are not included.