3V00

Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (C, B, A)	Guanine nucleotide-binding protein G(t) subunit alpha-1/ Guanine nucleotide-binding protein G(i) subunit alpha-1 chimeric protein	polymer	356	40999.7	3	UniProt (P04695) UniProt (P10824) Pfam (PF00503)	Bos taurus (bovine cow domestic cattle domestic cow)	Transducin alpha-1 chain
2	D, E, F (C, B, A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	3	Chemie (GDP)
3	G, H, I (C, B, A)	water	water		18.0	295	Chemie (HOH)

Sequence modifications

C, B, A: 1 - 215 (UniProt: P04695)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Pro 56	Gly 56	engineered mutation

C, B, A: 216 - 294 (UniProt: P10824)

PDB	External Database	Details
His 244	Lys 248	engineered mutation
Asn 247	Asp 251	engineered mutation

C, B, A: 295 - 350 (UniProt: P04695)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	122999.0
Non-Polymers*	Number of molecules	3
	Total formula weight	1329.6
All*	Total formula weight	124328.6

*Water molecules are not included.