3V00

Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (C, B, A)	Guanine nucleotide-binding protein G(t) subunit alpha-1/ Guanine nucleotide-binding protein G(i) subunit alpha-1 chimeric protein	polymer	356	40999.7	3	UniProt (P04695) UniProt (P10824) Pfam (PF00503)	Bos taurus (bovine cow domestic cattle domestic cow)	Transducin alpha-1 chain
2	D, E, F (C, B, A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	3	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
3	G, H, I (C, B, A)	water	water		18.0	295	Chemie (HOH)

Sequence modifications

C, B, A: 1 - 215 (UniProt: P04695)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Pro 56	Gly 56	engineered mutation

C, B, A: 216 - 294 (UniProt: P10824)

PDB	External Database	Details
His 244	Lys 248	engineered mutation
Asn 247	Asp 251	engineered mutation

C, B, A: 295 - 350 (UniProt: P04695)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	122999.0
Non-Polymers*	Number of molecules	3
	Total formula weight	1329.6
All*	Total formula weight	124328.6

*Water molecules are not included.