Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3V00

Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0019001	molecular_function	guanyl nucleotide binding
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
B	0003924	molecular_function	GTPase activity
B	0005525	molecular_function	GTP binding
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
B	0019001	molecular_function	guanyl nucleotide binding
B	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
C	0019001	molecular_function	guanyl nucleotide binding
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GDP C 401

Chain	Residue
C	GLY38
C	ARG172
C	ARG174
C	ASN265
C	LYS266
C	ASP268
C	LEU269
C	CYS321
C	ALA322
C	THR323
C	HOH515
C	GLU39
C	HOH519
C	SER40
C	GLY41
C	LYS42
C	SER43
C	THR44
C	SER147
C	LEU171

site_id	AC2
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GDP B 401

Chain	Residue
B	ALA37
B	GLY38
B	GLU39
B	SER40
B	GLY41
B	LYS42
B	SER43
B	THR44
B	SER147
B	LEU171
B	ARG172
B	ARG174
B	ASN265
B	LYS266
B	ASP268
B	LEU269
B	CYS321
B	ALA322
B	THR323

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE GDP A 401

Chain	Residue
A	GLU39
A	SER40
A	GLY41
A	LYS42
A	SER43
A	THR44
A	SER147
A	LEU171
A	ARG172
A	SER173
A	ARG174
A	ASN265
A	LYS266
A	ASP268
A	LEU269
A	CYS321
A	ALA322
A	THR323

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	966
Details	Domain: {"description":"G-alpha","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	40
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	39
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	24
Details	Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	27
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	21
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	15
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	30
Details	Region: {"description":"Interaction with RHO","evidences":[{"source":"PubMed","id":"18818650","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21389983","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21389988","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23579341","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9539726","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	28
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	42
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7969474","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8208289","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8259210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FQJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FQK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GOT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TAD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TAG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TND","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3V00","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19703466","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8259210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1TND","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	45
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	3
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P11488","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	2
Details	Lipidation: {"description":"N-myristoyl glycine","evidences":[{"source":"PubMed","id":"26253820","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	2
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"26253820","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 533

Chain	Residue	Details
A	GLU39	electrostatic stabiliser
A	THR44	electrostatic stabiliser
A	ARG174	electrostatic stabiliser
A	ASP196	electrostatic stabiliser
A	GLN200	electrostatic stabiliser

site_id	MCSA2
Number of Residues	5
Details	M-CSA 533

Chain	Residue	Details
B	GLU39	electrostatic stabiliser
B	THR44	electrostatic stabiliser
B	ARG174	electrostatic stabiliser
B	ASP196	electrostatic stabiliser
B	GLN200	electrostatic stabiliser

site_id	MCSA3
Number of Residues	5
Details	M-CSA 533

Chain	Residue	Details
C	GLU39	electrostatic stabiliser
C	THR44	electrostatic stabiliser
C	ARG174	electrostatic stabiliser
C	ASP196	electrostatic stabiliser
C	GLN200	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)