3UXV
Crystal Structure of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with NADP and PreQ
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | NADPH-dependent 7-cyano-7-deazaguanine reductase | polymer | 290 | 33283.3 | 4 | UniProt (C3LTF1) Pfam (PF14819) Pfam (PF14489) UniProt (by SIFTS) (Q9KTK0) In PDB | Vibrio cholerae O1 biovar El Tor | 7-cyano-7-carbaguanine reductase, NADPH-dependent nitrile oxidoreductase, PreQ(0) reductase |
| 2 | A, D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 3 | A, B, D | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 4 | B, D | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
| 5 | B, C | GUANINE | non-polymer | 151.1 | 2 | Chemie (GUN) | |||
| 6 | water | water | 18.0 | 832 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 287 (UniProt: C3LTF1)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | EXPRESSION TAG |
| Asn -1 | - | EXPRESSION TAG |
| Ala 0 | - | EXPRESSION TAG |
| Ala 194 | Cys 194 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 133133.0 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 2373.7 | |
| All* | Total formula weight | 135506.7 |
