3UXV
Crystal Structure of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with NADP and PreQ
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003674 | molecular_function | molecular_function |
| A | 0005575 | cellular_component | cellular_component |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0008150 | biological_process | biological_process |
| A | 0008616 | biological_process | queuosine biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0033739 | molecular_function | preQ1 synthase activity |
| A | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| B | 0003674 | molecular_function | molecular_function |
| B | 0005575 | cellular_component | cellular_component |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008150 | biological_process | biological_process |
| B | 0008616 | biological_process | queuosine biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0033739 | molecular_function | preQ1 synthase activity |
| B | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| C | 0003674 | molecular_function | molecular_function |
| C | 0005575 | cellular_component | cellular_component |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0008150 | biological_process | biological_process |
| C | 0008616 | biological_process | queuosine biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0033739 | molecular_function | preQ1 synthase activity |
| C | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| D | 0003674 | molecular_function | molecular_function |
| D | 0005575 | cellular_component | cellular_component |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0008150 | biological_process | biological_process |
| D | 0008616 | biological_process | queuosine biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0033739 | molecular_function | preQ1 synthase activity |
| D | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE EDO A 302 |
| Chain | Residue |
| A | HIS188 |
| A | SER204 |
| A | MSE284 |
| A | ALA285 |
| A | ARG286 |
| A | HOH311 |
| A | HOH371 |
| B | LEU139 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 303 |
| Chain | Residue |
| A | SER252 |
| A | SER273 |
| A | HIS274 |
| C | SER112 |
| C | ASP114 |
| C | HOH337 |
| A | GLN251 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE NAP B 301 |
| Chain | Residue |
| A | SER95 |
| A | LYS96 |
| A | LYS99 |
| A | GLN199 |
| A | ARG262 |
| A | GLY263 |
| B | SER95 |
| B | LYS96 |
| B | LYS99 |
| B | ARG262 |
| B | GLY263 |
| B | GUN300 |
| B | HOH325 |
| B | HOH686 |
| B | HOH719 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GUN B 300 |
| Chain | Residue |
| B | TRP62 |
| B | LEU92 |
| B | ILE93 |
| B | GLU94 |
| B | SER95 |
| B | PHE232 |
| B | HIS233 |
| B | GLU234 |
| B | ILE267 |
| B | NAP301 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL B 302 |
| Chain | Residue |
| B | ALA126 |
| B | CYS127 |
| B | HOH326 |
| B | HOH414 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GUN C 300 |
| Chain | Residue |
| C | TRP62 |
| C | LEU92 |
| C | ILE93 |
| C | GLU94 |
| C | SER95 |
| C | PHE232 |
| C | HIS233 |
| C | GLU234 |
| C | ILE267 |
| D | NAP301 |
| site_id | AC7 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE NAP D 301 |
| Chain | Residue |
| C | SER95 |
| C | LYS96 |
| C | LYS99 |
| C | ARG262 |
| C | GLY263 |
| C | LEU265 |
| C | GUN300 |
| D | SER95 |
| D | LYS96 |
| D | LYS99 |
| D | ARG262 |
| D | GLY263 |
| D | HOH332 |
| D | HOH717 |
| D | HOH718 |
| D | HOH824 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE EDO D 302 |
| Chain | Residue |
| C | LEU139 |
| D | HIS188 |
| D | SER204 |
| D | MSE284 |
| D | ALA285 |
| D | ARG286 |
| D | HOH293 |
| D | HOH466 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL D 303 |
| Chain | Residue |
| B | SER112 |
| B | TRP113 |
| B | ASP114 |
| D | GLN251 |
| D | SER252 |
| D | SER273 |
| D | HIS274 |
| site_id | BC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL D 304 |
| Chain | Residue |
| A | ASN91 |
| A | HOH289 |
| A | HOH369 |
| D | PRO38 |
| D | ASN91 |
| D | HOH312 |
| D | HOH393 |
| D | HOH471 |
| D | HOH607 |
| site_id | BC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL D 305 |
| Chain | Residue |
| D | HOH395 |
| D | HOH443 |
| D | HOH582 |
| D | GLU82 |
| D | GLU145 |
| D | ASN281 |
| D | GLN282 |
| D | HOH374 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Thioimide intermediate => ECO:0000255|HAMAP-Rule:MF_00817, ECO:0000269|Ref.3 |
| Chain | Residue | Details |
| B | ALA194 | |
| C | ALA194 | |
| D | ALA194 | |
| A | ALA194 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_00817 |
| Chain | Residue | Details |
| A | ASP201 | |
| B | ASP201 | |
| C | ASP201 | |
| D | ASP201 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| B | ILE93 | |
| B | SER95 | |
| B | HIS233 | |
| B | ARG262 | |
| C | ILE93 | |
| C | SER95 | |
| C | HIS233 | |
| C | ARG262 | |
| D | ILE93 | |
| D | SER95 | |
| D | HIS233 | |
| D | ARG262 | |
| A | ILE93 | |
| A | SER95 | |
| A | HIS233 | |
| A | ARG262 |
