3UVJ

Crystal structure of the catalytic domain of the heterodimeric human soluble guanylate cyclase 1.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Guanylate cyclase soluble subunit alpha-3	polymer	225	24713.5	2	UniProt (Q02108) Pfam (PF00211) In PDB	Homo sapiens (human)	GCS-alpha-3, GCS-alpha-1, Soluble guanylate cyclase large subunit
2	B, D	Guanylate cyclase soluble subunit beta-1	polymer	220	24818.2	2	UniProt (Q02153) Pfam (PF00211) In PDB	Homo sapiens (human)	GCS-beta-1, Guanylate cyclase soluble subunit beta-3, GCS-beta-3, Soluble guanylate cyclase small subunit
3	A, B, C	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
4	A, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	431	Chemie (HOH)

Sequence modifications

A, C: 468 - 690 (UniProt: Q02108)

PDB	External Database	Details
Ser 466	-	expression tag
Met 467	-	expression tag

B, D: 408 - 619 (UniProt: Q02153)

PDB	External Database	Details
Met 407	-	expression tag
Cys 476	Gly 476	engineered mutation
Ser 541	Cys 541	engineered mutation
Ala 620	-	expression tag
Glu 621	-	expression tag
Asn 622	-	expression tag
Leu 623	-	expression tag
Tyr 624	-	expression tag
Phe 625	-	expression tag
Gln 626	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	99063.4
Non-Polymers*	Number of molecules	7
	Total formula weight	494.5
All*	Total formula weight	99558.0

*Water molecules are not included.