3UVJ
Crystal structure of the catalytic domain of the heterodimeric human soluble guanylate cyclase 1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.968627 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.780, 139.100, 55.460 |
| Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
| Resolution | 51.500 - 2.080 |
| R-factor | 0.1688 |
| Rwork | 0.167 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.500 | 2.160 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 45874 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.6 | 94.5 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.05M KH2PO4 20% PEG 8000, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
