3UM5
Double mutant (A16V+S108T) Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS-T9/94) complexed with pyrimethamine, NADPH, and dUMP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Bifunctional dihydrofolate reductase-thymidylate synthase | polymer | 608 | 71870.1 | 2 | UniProt (A7UD81) Pfam (PF00186) Pfam (PF00303) | Plasmodium falciparum | DHFR-TS |
| 2 | C, F (A, B) | 5-(4-CHLORO-PHENYL)-6-ETHYL-PYRIMIDINE-2,4-DIAMINE | non-polymer | 248.7 | 2 | Chemie (CP6) | |||
| 3 | D, G (A, B) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 2 | Chemie (NDP) | |||
| 4 | E, H (A, B) | 2'-DEOXYURIDINE 5'-MONOPHOSPHATE | non-polymer | 308.2 | 2 | Chemie (UMP) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 781 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 608 (UniProt: A7UD81)
| PDB | External Database | Details |
|---|---|---|
| Val 16 | Ala 16 | engineered mutation |
| Thr 108 | Ser 108 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 143740.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2604.6 | |
| All* | Total formula weight | 146344.8 |
