3U9S

Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC) 750 kD holoenzyme, CoA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Methylcrotonyl-CoA carboxylase, alpha-subunit	polymer	655	71378.9	6	UniProt (Q9I299) Pfam (PF00289) Pfam (PF02786) Pfam (PF02785) Pfam (PF21139) Pfam (PF00364)	Pseudomonas aeruginosa	MCC alpha
2	B, D, F, H, J... (B, D, F, H, J...)	Methylcrotonyl-CoA carboxylase, beta-subunit	polymer	555	59670.0	6	UniProt (Q9I297) Pfam (PF01039)	Pseudomonas aeruginosa	MCC beta
3	M, R (A, I)	5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL	non-polymer		228.3	2	Chemie (BTI)
4	N, O, P, Q, S... (B, D, F, H, J...)	COENZYME A	non-polymer		767.5	6	Chemie (COA)

Sequence modifications

B, D, F, H, J, L: 28 - 563 (UniProt: Q9I297)

PDB	External Database	Details
Met 8	-	expression tag
Gly 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
Ser 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Leu 21	-	expression tag
Val 22	-	expression tag
Pro 23	-	expression tag
Arg 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag
His 27	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	786293.3
Non-Polymers*	Number of molecules	8
	Total formula weight	5061.8
All*	Total formula weight	791355.1

*Water molecules are not included.