3U61

Structure of T4 Bacteriophage Clamp Loader Bound To Closed Clamp, DNA and ATP Analog and ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, C, D, E	DNA polymerase accessory protein 44	polymer	324	36189.6	4	UniProt (P04526) Pfam (PF00004) Pfam (PF21328) Pfam (PF21429) In PDB	Enterobacteria phage T4	Clamp loader large subunit, Protein Gp44
2	A	DNA polymerase accessory protein 62	polymer	199	22941.3	1	UniProt (P04527) Pfam (PF16790) In PDB	Enterobacteria phage T4	Clamp loader small subunit, Protein Gp62
3	G, H, F	DNA polymerase processivity component	polymer	228	25162.6	3	UniProt (P04525) Pfam (PF02916) Pfam (PF09116) In PDB	Enterobacteria phage T4	DNA polymerase accessory protein 45, Gp45
4	I	Template DNA strand	polymer	20	6117.9	1
5	J	Primer DNA strand	polymer	10	3014.0	1
6	B	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
7	B, C, D	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	C, D	[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium	non-polymer		492.2	2	Chemie (08T)

Sequence modifications

B, C, D, E: 1 - 319 (UniProt: P04526)

PDB	External Database	Details
Gly -4	-	EXPRESSION TAG
Pro -3	-	EXPRESSION TAG
Gly -2	-	EXPRESSION TAG
Gly -1	-	EXPRESSION TAG
Ser 0	-	EXPRESSION TAG

A: 2 - 187 (UniProt: P04527)

PDB	External Database	Details
Gly 188	-	EXPRESSION TAG
Leu 189	-	EXPRESSION TAG
Glu 190	-	EXPRESSION TAG
His 191	-	EXPRESSION TAG
His 192	-	EXPRESSION TAG
His 193	-	EXPRESSION TAG
His 194	-	EXPRESSION TAG
His 195	-	EXPRESSION TAG
His 196	-	EXPRESSION TAG
His 197	-	EXPRESSION TAG
His 198	-	EXPRESSION TAG
His 199	-	EXPRESSION TAG
His 200	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	252319.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1484.5
All*	Total formula weight	253803.9

*Water molecules are not included.