3U0K

Crystal Structure of the genetically encoded calcium indicator RCaMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	RCaMP	polymer	213	23279.5	1	UniProt (K4DIE3) Pfam (PF01353) In PDB	Entacmaea quadricolor (Bubble-tip anemone)
2	A	RCaMP	polymer	229	26048.9	1	UniProt (K4DIE3) Pfam (PF01353) Pfam (PF13499) In PDB	Entacmaea quadricolor (Bubble-tip anemone)
3	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
4	A	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5		water	water		18.0	138	Chemie (HOH)

Sequence modifications

B: 11 - 213 (UniProt: K4DIE3)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Gly 10	-	expression tag

A: 214 - 442 (UniProt: K4DIE3)

PDB	External Database	Details
Crk 214	Met 204	chromophore
Crk 214	Tyr 205	chromophore
Crk 214	Gly 206	chromophore

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49328.4
Non-Polymers*	Number of molecules	5
	Total formula weight	219.4
All*	Total formula weight	49547.8

*Water molecules are not included.