3U0K

Crystal Structure of the genetically encoded calcium indicator RCaMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-02
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	75.918, 75.918, 123.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.580 - 2.100
R-factor	0.19181
Rwork	0.189
R-free	0.23852
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uis
RMSD bond length	0.022
RMSD bond angle	1.899
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0111)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.820	2.210
High resolution limit [Å]	2.100	2.100
Number of reflections	24607
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K