3TZE

Crystal structure of a tryptophanyl-tRNA synthetase from Encephalitozoon cuniculi bound to tryptophan

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tryptophanyl-tRNA synthetase	polymer	406	46571.1	2	UniProt (O96771) Pfam (PF00579)	Encephalitozoon cuniculi (Microsporidian parasite)	Tryptophan--tRNA ligase, TrpRS
2	C, E (A, B)	TRYPTOPHAN	non-polymer		204.2	2	Chemie (TRP)
3	D, F (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
4	G, H (A, B)	water	water		18.0	61	Chemie (HOH)

Sequence modifications

A, B: 1 - 385 (UniProt: O96771)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Met -12	-	expression tag
Gly -11	-	expression tag
Thr -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Ala -7	-	expression tag
Gln -6	-	expression tag
Thr -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Thr 149	Ala 149	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93142.3
Non-Polymers*	Number of molecules	4
	Total formula weight	486.6
All*	Total formula weight	93628.9

*Water molecules are not included.