3TWI

Variable Lymphocyte Receptor Recognition of the Immunodominant Glycoprotein of Bacillus anthracis Spores

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	BclA protein	polymer	160	16422.6	3	UniProt (Q81JD7) Pfam (PF18573)	Bacillus anthracis (anthrax anthrax bacterium)	Spore surface glycoprotein BclA
2	D, E, F (D, E, F)	Variable lymphocyte receptor B	polymer	179	19471.3	3	UniProt (A9Z0I5) Pfam (PF01462) Pfam (PF13855)	Petromyzon marinus (marine lamprey)
3	G, H, I (A, B, D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	J, K, L, M, N... (A, B, C, D, E...)	water	water		18.0	383	Chemie (HOH)

Sequence modifications

A, B, C: 77 - 214 (UniProt: Q81JD7)

PDB	External Database	Details
Met 60	-	expression tag
Gly 61	-	expression tag
Ser 62	-	expression tag
Ser 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
Glu 70	-	expression tag
Asn 71	-	expression tag
Leu 72	-	expression tag
Tyr 73	-	expression tag
Phe 74	-	expression tag
Gln 75	-	expression tag
Gly 76	-	expression tag
Asn 185	Thr 353	engineered mutation
Phe 215	-	expression tag
Arg 216	-	expression tag
Phe 217	-	expression tag
Arg 218	-	expression tag
Asn 219	-	expression tag

D, E, F: 21 - 188 (UniProt: A9Z0I5)

PDB	External Database	Details
Ala 10	-	expression tag
Met 11	-	expression tag
Val 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Ser 19	-	expression tag
Ala 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	107681.5
Non-Polymers*	Number of molecules	3
	Total formula weight	276.3
All*	Total formula weight	107957.8

*Water molecules are not included.