3TWI
Variable Lymphocyte Receptor Recognition of the Immunodominant Glycoprotein of Bacillus anthracis Spores
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 74.809, 146.528, 209.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.880 - 2.550 |
R-factor | 0.19149 |
Rwork | 0.189 |
R-free | 0.23293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2R6Q 2r9u |
RMSD bond length | 0.011 |
RMSD bond angle | 1.211 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (for MR) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 37042 | |
<I/σ(I)> | 9.5 | 2 |
Completeness [%] | 97.3 | 99.1 |
Redundancy | 3.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.26 | 298 | 15% PEG 6000, 1.2 M LiCl, 0.1 M sodium citrate, pH 5.26, VAPOR DIFFUSION, SITTING DROP, temperature 298K |