3TW6

Structure of Rhizobium etli pyruvate carboxylase T882A with the allosteric activator, acetyl coenzyme-A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Pyruvate carboxylase protein	polymer	1165	127532.0	4	UniProt (Q2K340) Pfam (PF00289) Pfam (PF02786) Pfam (PF02785) Pfam (PF00682) Pfam (PF02436) Pfam (PF00364) In PDB	Rhizobium etli
2	D, A, B, C	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
3	D, A, B, C	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
4	D, A, B, C	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	D, A, B, C	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
6	A, C	COENZYME A	non-polymer		767.5	2	Chemie (COA)
7	B	5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL	non-polymer		228.3	1	Chemie (BTI)
8	C	PHOSPHONOACETIC ACID	non-polymer		140.0	1	Chemie (PAE)
9		water	water		18.0	793	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 1154 (UniProt: Q2K340)

PDB	External Database	Details
Met -10	-	EXPRESSION TAG
His -9	-	EXPRESSION TAG
His -8	-	EXPRESSION TAG
His -7	-	EXPRESSION TAG
His -6	-	EXPRESSION TAG
His -5	-	EXPRESSION TAG
His -4	-	EXPRESSION TAG
His -3	-	EXPRESSION TAG
His -2	-	EXPRESSION TAG
His -1	-	EXPRESSION TAG
Gly 0	-	EXPRESSION TAG
Gly 1	-	EXPRESSION TAG
Ala 882	Thr 882	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	510128.0
Non-Polymers*	Number of molecules	27
	Total formula weight	4327.6
All*	Total formula weight	514455.6

*Water molecules are not included.