3TV8

Pharmacological Chaperoning in Human alpha-Galactosidase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Alpha-galactosidase A	polymer	404	46179.4	2	UniProt (P06280) Pfam (PF16499) Pfam (PF17450) In PDB	Homo sapiens (human)	Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Melibiase
2	C	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
3	D	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
4	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	A, B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	A, B	NONAETHYLENE GLYCOL	non-polymer		414.5	3	Chemie (2PE)
7	A, B	(2R,3S,4R,5S)-2-(hydroxymethyl)piperidine-3,4,5-triol	non-polymer		163.2	2	Chemie (DGJ)
8		water	water		18.0	64	Chemie (HOH)

Sequence modifications

A, B: 32 - 429 (UniProt: P06280)

PDB	External Database	Details
Ala 170	Asp 170	engineered mutation
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92358.8
Branched	Number of molecules	2
	Total formula weight	1335.2
Non-Polymers*	Number of molecules	12
	Total formula weight	2742.8
All*	Total formula weight	96436.9

*Water molecules are not included.