3TV1

Crystal structure of RtcA.AMP product complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RNA 3'-terminal phosphate cyclase	polymer	358	38304.5	2	UniProt (P46849) Pfam (PF01137) Pfam (PF05189) In PDB	Escherichia coli	RNA cyclase, RNA-3'-phosphate cyclase
2	A, B	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
3	A, B	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8		water	water		18.0	731	Chemie (HOH)

Sequence modifications

A, B: 2 - 339 (UniProt: P46849)

PDB	External Database	Details
Gly -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
His -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
His 1	-	expression tag
Ser 308	Cys 307	engineered mutation
Asn 309	His 308	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76609.1
Non-Polymers*	Number of molecules	10
	Total formula weight	1374.4
All*	Total formula weight	77983.5

*Water molecules are not included.