3TOI
Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Ampicillin resistance protein | polymer | 254 | 27699.5 | 2 | UniProt (Q79DR3) Pfam (PF00144) Pfam (PF13354) UniProt (by SIFTS) (P62593) In PDB | Escherichia coli | Beta-lactamase, Betalactamase TEM-116, Extended spectrum beta-lactamase, Mutant extended-spectrum beta-lactamase, TEM extended spectrum beta-lactamase |
2 | water | water | 18.0 | 250 | Chemie (HOH) |
Sequence modifications
A, B: 41 - 287 (UniProt: Q79DR3)
PDB | External Database | Details |
---|---|---|
Asp 39 | - | expression tag |
Gly 40 | - | expression tag |
Val 56 | Ile 54 | engineered mutation |
Gly 120 | Arg 118 | engineered mutation |
Thr 182 | Met 180 | engineered mutation |
Ser 195 | Thr 193 | engineered mutation |
Met 208 | Ile 206 | engineered mutation |
Val 224 | Ala 222 | engineered mutation |
His 241 | Arg 238 | engineered mutation |
Met 265 | Thr 261 | engineered mutation |
Gly 288 | - | expression tag |
Gly 289 | - | expression tag |
His 290 | - | expression tag |
His 291 | - | expression tag |
His 292 | - | expression tag |
His 293 | - | expression tag |
His 294 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 55398.9 | |
All* | Total formula weight | 55398.9 |