3TMH

Crystal structure of dual-specific A-kinase anchoring protein 2 in complex with cAMP-dependent protein kinase A type II alpha and PDZK1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (A, E, I)	Na(+)/H(+) exchange regulatory cofactor NHE-RF3	polymer	87	9277.6	3	UniProt (Q5T2W1) Pfam (PF00595)	Homo sapiens (human)	NHERF-3, CFTR-associated protein of 70 kDa, Na(+)/H(+) exchanger regulatory factor 3, Na/Pi cotransporter C-terminal-associated protein 1, NaPi-Cap1, PDZ domain-containing protein 1, Sodium-hydrogen exchanger regulatory factor 3
2	B, C, F, G (B, C, F, G)	cAMP-dependent protein kinase type II-alpha regulatory subunit	polymer	48	5542.3	4	UniProt (P12368) Pfam (PF02197)	Rattus norvegicus (brown rat rat rats)
3	D, H, J (D, H, L)	A-kinase anchor protein 10, mitochondrial	polymer	45	5086.7	3	UniProt (O43572)	Homo sapiens (human)	AKAP-10, Dual specificity A kinase-anchoring protein 2, D-AKAP-2, Protein kinase A-anchoring protein 10, PRKA10
4	K, L, M, N, O (A, D, E, F, I)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, E, I: 375 - 459 (UniProt: Q5T2W1)

PDB	External Database	Details
Gly 373	-	expression tag
Ser 374	-	expression tag

B, C, F, G: 0 - 44 (UniProt: P12368)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Gly 1	Ser 2	conflict

D, H, L: 623 - 662 (UniProt: O43572)

PDB	External Database	Details
Gly 618	-	expression tag
Ser 619	-	expression tag
Pro 620	-	expression tag
Glu 621	-	expression tag
Phe 622	-	expression tag
Val 646	Ile 646	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	65262.1
All*	Total formula weight	65262.1

*Water molecules are not included.