3TMH
Crystal structure of dual-specific A-kinase anchoring protein 2 in complex with cAMP-dependent protein kinase A type II alpha and PDZK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2010-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.472, 121.472, 88.282 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.038 - 3.800 |
| R-factor | 0.2727 |
| Rwork | 0.268 |
| R-free | 0.32840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.710 | 0.477 |
| Number of reflections | 8304 | |
| <I/σ(I)> | 16.9 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 293 | 100 mM HEPES, pH 7, 20% PEG MME, Microbatch, temperature 293K |
