3TLY

Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	MccF	polymer	371	41615.5	2	UniProt (Q2KKH9) Pfam (PF02016) Pfam (PF17676) UniProt (by SIFTS) (Q47511) In PDB	Escherichia coli
2	A, B	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
3		water	water		18.0	920	Chemie (HOH)

Sequence modifications

A, B: 2 - 344 (UniProt: Q2KKH9)

PDB	External Database	Details
Met -26	-	expression tag
Gly -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
His -9	-	expression tag
Ile -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Leu 0	-	expression tag
Glu 1	-	expression tag
Ala 118	Ser 118	engineered mutation
Ala 220	Asn 220	engineered mutation
Ala 247	Lys 247	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83230.9
Non-Polymers*	Number of molecules	6
	Total formula weight	372.4
All*	Total formula weight	83603.3

*Water molecules are not included.