3TLY
Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-04 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.422, 85.658, 73.016 |
Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.700 |
R-factor | 0.1731 |
Rwork | 0.172 |
R-free | 0.20080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tla |
RMSD bond length | 0.006 |
RMSD bond angle | 0.988 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
High resolution limit [Å] | 1.690 | 4.590 | 1.690 |
Rmerge | 0.063 | 0.048 | 0.210 |
Number of reflections | 73757 | ||
<I/σ(I)> | 13.7 | ||
Completeness [%] | 100.0 | 99.4 | 99.9 |
Redundancy | 6.1 | 6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | 10% PEG 8000, 8% ethylene glycol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 282K |