3T3O

Molecular basis for the recognition and cleavage of RNA (CUGG) by the bifunctional 5'-3' exo/endoribonuclease RNase J

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Metal dependent hydrolase	polymer	562	62612.4	1	UniProt (Q72JJ7) Pfam (PF12706) Pfam (PF22505) Pfam (PF07521) Pfam (PF17770) In PDB	Thermus thermophilus HB27
2	B	O2'methyl-RNA	polymer	5	1619.1	1
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5		water	water		18.0	97	Chemie (HOH)

Sequence modifications

A: 2 - 554 (UniProt: Q72JJ7)

PDB	External Database	Details
Met -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 1	-	expression tag
Ala 77	His 95	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64231.5
Non-Polymers*	Number of molecules	2
	Total formula weight	157.5
All*	Total formula weight	64389.0

*Water molecules are not included.