3T13
Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Thermonuclease | polymer | 143 | 16129.4 | 2 | UniProt (P00644) Pfam (PF00565) | Staphylococcus aureus | TNase, Micrococcal nuclease, Staphylococcal nuclease |
| 2 | C, D, H (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) | |||
| 3 | E, K (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | F, G, I, J (A, B) | (4R)-2-METHYLPENTANE-2,4-DIOL | non-polymer | 118.2 | 4 | Chemie (MRD) | |||
| 5 | L, M (A, B) | water | water | 18.0 | 266 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 149 (UniProt: P00644)
| PDB | External Database | Details |
|---|---|---|
| - | Thr 126 | deletion |
| - | Lys 127 | deletion |
| - | His 128 | deletion |
| - | Pro 129 | deletion |
| - | Lys 130 | deletion |
| - | Lys 131 | deletion |
| Phe 50 | Gly 132 | engineered mutation |
| Asn 51 | Val 133 | engineered mutation |
| Gly 69 | Ala 151 | engineered mutation |
| Gly 117 | Pro 199 | engineered mutation |
| Leu 124 | His 206 | engineered mutation |
| Ala 128 | Ser 210 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 32258.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 837.8 | |
| All* | Total formula weight | 33096.6 |
