3T12

MglA in complex with MglB in transition state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Gliding protein mglA	polymer	198	21999.3	1	UniProt (Q5SJ82) Pfam (PF00025)	Thermus thermophilus
2	B, C (B, C)	Gliding protein MglB	polymer	136	14326.4	2	UniProt (Q5SJ83) Pfam (PF03259)	Thermus thermophilus
3	D (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
4	E, F, H (A, B)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	G (A)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	1	Chemie (ALF)
6	I, J, K (A, B, C)	water	water		18.0	192	Chemie (HOH)

Sequence modifications

A: 1 - 196 (UniProt: Q5SJ82)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

B, C: 6 - 139 (UniProt: Q5SJ83)

PDB	External Database	Details
Gly 4	-	expression tag
Ser 5	-	expression tag
Ala 14	Glu 14	engineered mutation
Ala 15	Arg 15	engineered mutation
Ser 65	Gly 65	variant
Ala 124	Arg 124	engineered mutation
Ala 127	Glu 127	engineered mutation
Ala 131	Arg 131	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	50652.1
Non-Polymers*	Number of molecules	5
	Total formula weight	619.1
All*	Total formula weight	51271.2

*Water molecules are not included.