3SPY

RB69 DNA Polymerase(L415A/L561A/S565G/Y567A) Ternary Complex with dUpCpp Opposite dA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase	polymer	901	104186.6	1	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136) In PDB	Enterobacteria phage RB69	Gp43
2	T	5'-D(*TP*CP*GP*AP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3'	polymer	18	5541.6	1
3	P	5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3'	polymer	13	3967.6	1
4	A	2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]uridine	non-polymer		466.2	1	Chemie (UPC)
5	A	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
6		water	water		18.0	494	Chemie (HOH)

Sequence modifications

A: 1 - 901 (UniProt: Q38087)

PDB	External Database	Details
Ala 222	Asp 222	engineered mutation
Ala 327	Asp 327	engineered mutation
Ala 415	Leu 415	engineered mutation
Ala 561	Leu 561	engineered mutation
Gly 565	Ser 565	engineered mutation
Ala 567	Tyr 567	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	113695.8
Non-Polymers*	Number of molecules	6
	Total formula weight	587.7
All*	Total formula weight	114283.5

*Water molecules are not included.