3SPY
RB69 DNA Polymerase(L415A/L561A/S565G/Y567A) Ternary Complex with dUpCpp Opposite dA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-06-11 |
Detector | APEX II CCD |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.336, 120.598, 130.812 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.670 - 2.140 |
R-factor | 0.19357 |
Rwork | 0.191 |
R-free | 0.24205 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.147 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.670 | 2.230 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 61948 | |
Completeness [%] | 94.5 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 150 mM calcium chloride, 15% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 298K |