3SPL

Crystal structure of aprataxin ortholog Hnt3 in complex with DNA and AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Aprataxin-like protein	polymer	204	23608.1	4	UniProt (O74859) Pfam (PF01230) Pfam (PF16278) In PDB	Schizosaccharomyces pombe (Fission yeast)	Hnt3 protein, Hit family protein 3
2	E, G	DNA (5'-D(*TP*AP*TP*TP*CP*CP*GP*AP*TP*AP*GP*TP*GP*AP*C)-3')	polymer	15	4584.0	2
3	F, H	DNA (5'-D(*GP*TP*CP*AP*CP*TP*AP*TP*CP*GP*GP*AP*AP*TP*GP*AP*G)-3')	polymer	17	5251.4	2
4	D, A, B, C	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	D, A, B, C	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	4	Chemie (AMP)
6	D, A, B, C	SULFATE ION	non-polymer		96.1	16	Chemie (SO4)
7		water	water		18.0	842	Chemie (HOH)

Sequence modifications

A, B, C, D: 33 - 232 (UniProt: O74859)

PDB	External Database	Details
Gly 29	-	expression tag
Ser 30	-	expression tag
His 31	-	expression tag
Met 32	-	expression tag
Ala 130	Cys 130	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	114103.1
Non-Polymers*	Number of molecules	24
	Total formula weight	3187.5
All*	Total formula weight	117290.6

*Water molecules are not included.