3SN6

Crystal structure of the beta2 adrenergic receptor-Gs protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44370.2	1	UniProt (P04896) Pfam (PF00503) In PDB	Bos taurus (Bovine)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Rat)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I
4	R	Endolysin,Beta-2 adrenergic receptor	polymer	514	58303.1	1	UniProt (P00720) UniProt (P07550) Pfam (PF00959) Pfam (PF00001) In PDB	Enterobacteria phage T4	Lysis protein,Lysozyme,Muramidase,Beta-2 adrenoreceptor,Beta-2 adrenoceptor
5	N	Camelid antibody VHH fragment	polymer	138	15140.7	1		Lama glama
6	R	8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one	non-polymer		370.4	1	Chemie (P0G)

Sequence modifications

A: 1 - 394 (UniProt: P04896)

PDB	External Database	Details
Ser 86	Gly 72	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Asp 987	-	expression tag
Tyr 988	-	expression tag
Lys 989	-	expression tag
Asp 990	-	expression tag
Asp 991	-	expression tag
Asp 992	-	expression tag
Asp 993	-	expression tag
Ala 994	-	expression tag
Glu 995	-	expression tag
Asn 996	-	expression tag
Leu 997	-	expression tag
Tyr 998	-	expression tag
Phe 999	-	expression tag
Gln 1000	-	expression tag
Gly 1001	-	expression tag
Gly 1012	Arg 12	conflict
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	conflict
Ala	-	linker
Ala	-	linker

R: 29 - 365 (UniProt: P07550)

PDB	External Database	Details
Thr 96	Met 96	engineered mutation
Thr 98	Met 98	engineered mutation
Glu 187	Asn 187	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	164122.1
Non-Polymers*	Number of molecules	1
	Total formula weight	370.4
All*	Total formula weight	164492.6

*Water molecules are not included.