3SK6

Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Thermonuclease	polymer	143	16182.6	1	UniProt (P00644) Pfam (PF00565) In PDB	Staphylococcus aureus	TNase, Micrococcal nuclease, Staphylococcal nuclease
2	A	THYMIDINE-3',5'-DIPHOSPHATE	non-polymer		402.2	1	Chemie (THP)
3	A	(4R)-2-METHYLPENTANE-2,4-DIOL	non-polymer		118.2	1	Chemie (MRD)
4		water	water		18.0	117	Chemie (HOH)

Sequence modifications

A: 1 - 149 (UniProt: P00644)

PDB	External Database	Details
Asn 21	Asp 103	engineered mutation
Lys 23	Val 105	engineered mutation
Val 33	Thr 115	engineered mutation
Glu 36	Leu 118	engineered mutation
Ile 41	Thr 123	engineered mutation
-	Thr 126	deletion
-	Lys 127	deletion
-	His 128	deletion
-	Pro 129	deletion
-	Lys 130	deletion
-	Lys 131	deletion
Phe 50	Gly 132	engineered mutation
Asn 51	Val 133	engineered mutation
Ala 59	Ser 141	engineered mutation
Gly 117	Pro 199	engineered mutation
Leu 124	His 206	engineered mutation
Ala 128	Ser 210	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	16182.6
Non-Polymers*	Number of molecules	2
	Total formula weight	520.4
All*	Total formula weight	16702.9

*Water molecules are not included.