3SK6
Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-14 |
Detector | BRUKER APEX II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.012, 60.472, 79.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.120 - 1.800 |
R-factor | 0.1998 |
Rwork | 0.198 |
R-free | 0.23770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.016 |
RMSD bond angle | 1.493 |
Data reduction software | XPREP (2008/2 for Windows) |
Phasing software | PHASER (2.2.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.120 | 33.120 | 1.830 |
High resolution limit [Å] | 1.800 | 5.110 | 1.800 |
Rmerge | 0.047 | 0.014 | 0.370 |
Number of reflections | 14455 | 724 | 465 |
<I/σ(I)> | 19.51 | ||
Completeness [%] | 100.0 | 99.3 | 100 |
Redundancy | 21.79 | 35.41 | 9.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |