3SIO
Ac-AChBP ligand binding domain (not including beta 9-10 linker) mutated to human alpha-7 nAChR
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Soluble acetylcholine receptor | polymer | 230 | 26238.0 | 10 | UniProt (Q8WSF8) Pfam (PF02931) | Aplysia californica (California sea hare) | |
| 2 | K, M, O (K, M, O) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 3 | L, P (L, P) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 2 | In PDB GlyTouCan (G55220VL) | |||
| 4 | N, Q (N, Q) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 5 | CA, EA, IA, LA, PA... (E, F, H, I, J...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 7 | Chemie (NAG) | |||
| 6 | AA, DA, FA, HA, KA... (D, E, F, G, H...) | METHYLLYCACONITINE | non-polymer | 682.8 | 10 | Chemie (MLK) | |||
| 7 | BA, GA, JA, OA, T... (D, G, H, I, B...) | (4R)-2-METHYLPENTANE-2,4-DIOL | non-polymer | 118.2 | 7 | Chemie (MRD) | |||
| 8 | MA, Y (I, C) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 2 | Chemie (MPD) | |||
| 9 | AB, RA, SA, TA, UA... (J, A, B, C, D...) | water | water | 18.0 | 832 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H, I, J: 1 - 219 (UniProt: Q8WSF8)
| PDB | External Database | Details |
|---|---|---|
| Asp -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Lys -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Tyr 32 | Thr 49 | engineered mutation |
| Ser 34 | Gly 51 | engineered mutation |
| Ser 36 | Thr 53 | engineered mutation |
| Leu 38 | Gln 55 | engineered mutation |
| Trp 55 | Tyr 72 | engineered mutation |
| Ser 59 | Arg 76 | engineered mutation |
| Asn 106 | Ile 123 | engineered mutation |
| Leu 108 | Val 125 | engineered mutation |
| Asn 110 | Thr 127 | engineered mutation |
| Ser 111 | His 128 | engineered mutation |
| Ser 112 | Asp 129 | engineered mutation |
| His 114 | Ser 131 | engineered mutation |
| Gln 116 | Met 133 | engineered mutation |
| Tyr 117 | Phe 134 | engineered mutation |
| Leu 118 | Ile 135 | engineered mutation |
| Ser 148 | Val 165 | engineered mutation |
| Gly 150 | Ser 167 | engineered mutation |
| Trp 152 | Phe 169 | engineered mutation |
| Ser 220 | - | expression tag |
| Arg 221 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 262379.7 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 5889.3 | |
| Non-Polymers* | Number of molecules | 26 |
| Total formula weight | 9440.0 | |
| All* | Total formula weight | 277709.1 |
