3SFV

Crystal structure of the GDP-bound Rab1a S25N mutant in complex with the coiled-coil domain of LidA from Legionella pneumophila

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ras-related protein Rab-1A	polymer	181	20466.2	1	UniProt (P62820) Pfam (PF00071)	Homo sapiens (human)	YPT1-related protein
2	B (B)	LidA protein, substrate of the Dot/Icm system	polymer	344	40201.6	1	UniProt (A5IFX1)	Legionella pneumophila
3	C (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
4	D, E (A, B)	water	water		18.0	389	Chemie (HOH)

Sequence modifications

A: 1 - 176 (UniProt: P62820)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 25	Ser 25	engineered mutation

B: 224 - 559 (UniProt: A5IFX1)

PDB	External Database	Details
Leu 560	-	expression tag
Glu 561	-	expression tag
His 562	-	expression tag
His 563	-	expression tag
His 564	-	expression tag
His 565	-	expression tag
His 566	-	expression tag
His 567	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60667.8
Non-Polymers*	Number of molecules	1
	Total formula weight	443.2
All*	Total formula weight	61111.0

*Water molecules are not included.