3SCX

RB69 DNA Polymerase Triple Mutant(L561A/S565G/Y567A) Ternary Complex with dUpNpp and a Deoxy-terminated Primer in the Presence of Ca2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (T)	5'-D(*TP*CP*AP*AP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3'	polymer	18	5525.6	1
2	B (P)	5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3'	polymer	13	3967.6	1
3	C (A)	DNA polymerase	polymer	903	104446.9	1	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136)	Enterobacteria phage RB69	Gp43
4	D (A)	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DUP)
5	E, F, G, H (A)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	I, J, K (T, P, A)	water	water		18.0	345	Chemie (HOH)

Sequence modifications

A: 1 - 903 (UniProt: Q38087)

PDB	External Database	Details
Ala 222	Asp 222	engineered mutation
Ala 327	Asp 327	engineered mutation
Ala 561	Leu 561	engineered mutation
Gly 565	Ser 565	engineered mutation
Ala 567	Tyr 567	engineered mutation
Ala 902	Asp 902	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	113940.1
Non-Polymers*	Number of molecules	5
	Total formula weight	627.5
All*	Total formula weight	114567.6

*Water molecules are not included.