3SCX
RB69 DNA Polymerase Triple Mutant(L561A/S565G/Y567A) Ternary Complex with dUpNpp and a Deoxy-terminated Primer in the Presence of Ca2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.022, 119.333, 130.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.040 - 2.350 |
R-factor | 0.21627 |
Rwork | 0.214 |
R-free | 0.25875 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ig9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.168 | |
Number of reflections | 49563 | |
<I/σ(I)> | 9.23 | 1.08 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH VAPOR DIFFUSION | 6.5 | 293.15 | 150 mM calcium chloride, 1% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, MICROBATCH VAPOR DIFFUSION, temperature 293.15K |