3SB5
Zn-mediated Trimer of T4 Lysozyme R125C/E128C by Synthetic Symmetrization
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (B, A, C, D) | Lysozyme | polymer | 165 | 18532.3 | 4 | UniProt (P00720) Pfam (PF00959) | Enterobacteria phage T4 | Endolysin, Lysis protein, Muramidase |
| 2 | AA, BA, E, F, M... (D, B, A, C) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 3 | EA, G, IA, L, P... (D, B, A, C) | CHLORIDE ION | non-polymer | 35.5 | 8 | Chemie (CL) | |||
| 4 | DA, FA, H, I, Q... (D, B, A) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 5 | CA, GA, HA, J, K... (D, B, A, C) | GLYCEROL | non-polymer | 92.1 | 9 | Chemie (GOL) | |||
| 6 | JA, KA, LA, MA (B, A, C, D) | water | water | 18.0 | 191 | Chemie (HOH) |
Sequence modifications
B, A, C, D: 1 - 162 (UniProt: P00720)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Thr 54 | Cys 54 | engineered mutation |
| Ala 97 | Cys 97 | engineered mutation |
| Cys 125 | Arg 125 | engineered mutation |
| Cys 128 | Glu 128 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 74129.2 | |
| Non-Polymers* | Number of molecules | 31 |
| Total formula weight | 1781.6 | |
| All* | Total formula weight | 75910.8 |
