Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3SB5

Zn-mediated Trimer of T4 Lysozyme R125C/E128C by Synthetic Symmetrization

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003796	molecular_function	lysozyme activity
A	0003824	molecular_function	catalytic activity
A	0009253	biological_process	peptidoglycan catabolic process
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0016998	biological_process	cell wall macromolecule catabolic process
A	0030430	cellular_component	host cell cytoplasm
A	0031640	biological_process	killing of cells of another organism
A	0042742	biological_process	defense response to bacterium
A	0044659	biological_process	viral release from host cell by cytolysis
B	0003796	molecular_function	lysozyme activity
B	0003824	molecular_function	catalytic activity
B	0009253	biological_process	peptidoglycan catabolic process
B	0016787	molecular_function	hydrolase activity
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0016998	biological_process	cell wall macromolecule catabolic process
B	0030430	cellular_component	host cell cytoplasm
B	0031640	biological_process	killing of cells of another organism
B	0042742	biological_process	defense response to bacterium
B	0044659	biological_process	viral release from host cell by cytolysis
C	0003796	molecular_function	lysozyme activity
C	0003824	molecular_function	catalytic activity
C	0009253	biological_process	peptidoglycan catabolic process
C	0016787	molecular_function	hydrolase activity
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0016998	biological_process	cell wall macromolecule catabolic process
C	0030430	cellular_component	host cell cytoplasm
C	0031640	biological_process	killing of cells of another organism
C	0042742	biological_process	defense response to bacterium
C	0044659	biological_process	viral release from host cell by cytolysis
D	0003796	molecular_function	lysozyme activity
D	0003824	molecular_function	catalytic activity
D	0009253	biological_process	peptidoglycan catabolic process
D	0016787	molecular_function	hydrolase activity
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0016998	biological_process	cell wall macromolecule catabolic process
D	0030430	cellular_component	host cell cytoplasm
D	0031640	biological_process	killing of cells of another organism
D	0042742	biological_process	defense response to bacterium
D	0044659	biological_process	viral release from host cell by cytolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 163

Chain	Residue
A	CYS125
A	CYS125
A	CYS128
A	CL166

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 164

Chain	Residue
A	CL169
A	CYS128
A	CYS128
A	CYS128
A	CL169
A	CL169

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 163

Chain	Residue
D	CYS125
D	CYS125
D	CYS128
D	CL167

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN D 164

Chain	Residue
D	CYS128
D	CYS128
D	CYS128
D	CL171
D	CL171
D	CL171

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN B 163

Chain	Residue
B	CYS128
B	CYS128
B	CYS128
B	CL170
B	CL170
B	CL170

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 164

Chain	Residue
B	CYS125
B	CYS125
B	CYS128
B	CL165

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 163

Chain	Residue
C	CYS125
C	CYS125
C	CYS128
C	CL165

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN C 164

Chain	Residue
C	CYS128
C	CYS128
C	CYS128
C	CL167
C	CL167
C	CL167

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL C 165

Chain	Residue
C	GLN123
C	CYS125
C	ASP127
C	CYS128
C	ZN163

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 165

Chain	Residue
A	GLU11
A	ILE29
A	GLY30
A	ASP70
A	PHE104

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL B 165

Chain	Residue
B	CYS125
B	ASP127
B	CYS128
B	ZN164

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 166

Chain	Residue
A	GLN123
A	ASP127
A	CYS128
A	ZN163

site_id	BC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL D 165

Chain	Residue
C	TYR25
C	PRO37
C	SER38
C	LEU39
D	ILE9
D	ASP10
D	ASN144
D	ARG145
D	ARG148
D	MG166
D	HOH193
D	HOH223

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 166

Chain	Residue
B	THR142
B	ASN144
B	ARG145
B	GOL169

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 166

Chain	Residue
D	THR142
D	ASN144
D	ARG145
D	GOL165
D	HOH193

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 167

Chain	Residue
A	THR142
A	ASN144
A	ARG145

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL D 167

Chain	Residue
D	GLN123
D	CYS125
D	ASP127
D	CYS128
D	ZN163

site_id	BC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG B 167

Chain	Residue
B	HOH177

site_id	CC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 168

Chain	Residue
A	SER90
A	LEU91
A	LEU121
A	GLN122
A	LYS124
A	TRP126
A	HOH178
D	TRP126

site_id	CC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL B 168

Chain	Residue
B	LYS147
B	ILE150
B	THR151
B	HOH196
B	HOH224
B	ALA130
B	ALA134
B	TYR139

site_id	CC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG D 168

Chain	Residue
D	GLU11
D	HOH226
D	HOH227
D	HOH228
D	HOH229
D	HOH230
D	HOH231

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 169

Chain	Residue
D	TYR88
D	ALA93
D	ARG96
D	ILE100
D	HOH177

site_id	CC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 166

Chain	Residue
C	ILE29
C	GLY30
C	ASP70
C	PHE104

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 169

Chain	Residue
B	ASP10
B	THR142
B	ARG145
B	ARG148
B	MG166

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 170

Chain	Residue
C	LYS35
C	SER36
D	TYR18
D	LYS19
D	HOH212

site_id	CC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL D 171

Chain	Residue
D	CYS128
D	CYS128
D	ZN164
D	ZN164
D	ZN164

site_id	CC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL A 169

Chain	Residue
A	CYS128
A	CYS128
A	ZN164
A	ZN164
A	ZN164

site_id	DC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL B 170

Chain	Residue
B	ZN163
B	ZN163
B	ZN163

site_id	DC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL C 167

Chain	Residue
C	ZN164
C	ZN164
C	ZN164

site_id	DC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL A 170

Chain	Residue
A	ALA93

site_id	DC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 171

Chain	Residue
A	PHE114
A	SER117
A	ASN132
B	ARG137

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_04110","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"3382407","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7831309","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8266098","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_04110","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1892846","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3382407","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7831309","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8266098","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8266098","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7831309","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
A	GLU11	proton shuttle (general acid/base)
A	ASP20	covalent catalysis

site_id	MCSA2
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
B	GLU11	proton shuttle (general acid/base)
B	ASP20	covalent catalysis

site_id	MCSA3
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
C	GLU11	proton shuttle (general acid/base)
C	ASP20	covalent catalysis

site_id	MCSA4
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
D	GLU11	proton shuttle (general acid/base)
D	ASP20	covalent catalysis

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)