3SAM

Structure of D13, the scaffolding protein of vaccinia virus (mutant D513G)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Rifampicin resistance protein	polymer	576	64992.8	3	UniProt (P68440) Pfam (PF03340)	Vaccinia virus (VACV)	62 kDa protein
2	D, E, F, G, H... (A, C)	FORMIC ACID	non-polymer		46.0	7	Chemie (FMT)
3	K, L, M (A, B, C)	water	water		18.0	625	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 551 (UniProt: P68440)

PDB	External Database	Details
Met -24	-	expression tag
Ser -23	-	expression tag
Tyr -22	-	expression tag
Tyr -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Gly 513	Asp 513	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	194978.4
Non-Polymers*	Number of molecules	7
	Total formula weight	322.2
All*	Total formula weight	195300.6

*Water molecules are not included.